Biomedical Microdevices
Give an overview of the research conducted in the lab (microfab)
A Microfluidic Study of Aquaporin Response to Osmotic Stress at Single Cell Level.
Blood Coagulation Assay
A Microfluidic Assay For Single Cell Bacterial Adhesion Studies Under Shear Stress
Brain Spheroids Dynamics
Hydrodynamic Trap
Stem Cell Mechanobiology
dCFU
Aquaporins are a family of small integral membrane proteins that transport water across cell membranes in response to osmotic gradients. They facilitate fluid secretion and absorption across epithelial surfaces in kidney tubules, exocrine glands, and gastrointestinal tract. Here, we describe a novel microfluidic method to evaluate and screen for aquaporin-based transmembrane permeability in mammalian cells. A microfluidic device was designed and fabricated for the encapsulation of single mammalian and yeast cells in micron-sized droplets. For this purpose, Chinese Hamster Ovarian (CHO) cells were used. CHO cells express AQP1 (aquaporin-1) homologous to human kidney aquaporins. The cells were cultivated and exposed to different osmotic stresses to study the transmembrane water transport performance of aquaporins. Our microfluidic platform has the potential to screen for and isolate cells with best aquaporin water transport performance for a number of applications in bioengineering.
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The study of bacterial adhesion to host cells is important in understanding how bacterial pathogenesis occurs, and how novel treatment methods can be created. Studying bacterial adhesion under shear stress is essential when creating an in vitro simulation of physiologically relevant conditions. To mimic this attachment to the mucosal surface a novel method of testing and imaging using microfluidic devices was created.
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